Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Could Tylenol be making your child's health worse? Acetaminophen is widely used for pain and fever relief, but research suggests it depletes glutathione, a powerful antioxidant essential for immune function, Ferroptosis as a therapeutic target in glioblastoma: Mechanisms and emerging strategies ScienceDirect Jenny McCarthy's Glutathione and Tylenol Claims: Medical Toxicologist Weighs In MedPage Today Exposure to acetaminophen and all its metabolites upon 10, 15, and 20 mg kg intravenous acetaminophen in very preterm infants Pediatric Research