Frontiers Phytic acid attenuates acetaminophen induced hepatotoxicity via modulating iron mediated oxidative stress and SIRT 1 expression in mice Frontiers MitochondrialLysosomal Axis in Acetaminophen Hepatotoxicity g glutamyl cycle. Depletion of glutathione by acetaminophen leads to Download Scientific Diagram Acetaminophen toxicity EMCrit Project Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors.