Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Acetaminophen, also known as Tylenol, is used to help with pain & to reduce fever. However, taking too much acetaminophen can be harmful to the liver which filters out toxins & Is Tylenol Safe During Pregnancy? Risk Factors, Not Causation Metabolism of acetaminophen (paracetamol) in plantstwo independent pathways result in the formation of a glutathione and a glucose conjugate Environmental Science and Pollution Research Springer Nature Link Electrochemical evidences in oxidation of acetaminophen in the presence of glutathione and N acetylcysteine Chemical Communications (RSC Publishing)