Identification of cytotoxic, glutathione reactive moieties inducing accumulation of reactive oxygen species via glutathione depletion ScienceDirect Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. 5 Factors that Deplete Glutathione Deubiquitinases Maintain Protein Homeostasis and Survival of Cancer Cells upon Glutathione Depletion: Cell Metabolism Not only that, it depletes your #glutathione levels!