Protocol for purifying biologically active microtubule severing protein UNC 45A from E.coli using GST affinity and spin columns: STAR Protocols Sepharose 4B resin Cytiva Cancer associated fibroblasts (CAFs) derived from MFAP2 promote CRC proliferation and metastasis while suppressing CD8+ T cell mediated antitumor immunity Cell Death & Disease Glutathione S transferase (GST) Gene Fusion System Metabolic profiling reveals pyrimidine synthesis enzyme CAD as a central carbon metabolism signaling node in cancer cell proliferation ScienceDirect
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