Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Serotonin Deficiency Exacerbates Acetaminophen Induced Liver Toxicity In Mice Scientific Reports The Metabolism of Acetaminophen and the Synthesis of Glutathione Download Scientific Diagram Not only that, it depletes your #glutathione levels! Is Tylenol Safe During Pregnancy? Risk Factors, Not Causation