Role of Carnitine Shuttle System in the Active Transport of Fatty Acyl Coa into the mitochondrial Short Note On Carnitine Shuttle: Fatty acids are oxidized in mitochondria, and synthesized in the cytoplasm. Long chain fatty acids must form an active intermediate, fatty acyl CoA, before being oxidized inside mitochondria. Catherine Fatty Acid Transport an overview ScienceDirect Topics Fatty acid transport into mitochondria by carnitine transporter The Role of l Carnitine in Mitochondria, Prevention of Metabolic Inflexibility and Disease Initiation