Can Tylenol (acetaminophen) deplete glutathione levels in the body? Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Does Acetaminophen Deplete Glutathione? Understanding the Connection Cymbiotika CYMBIOTIKA Illustration of the effects of acetaminophen on glutathione, Download Scientific Diagram PGC 1 Induces Susceptibility To Acetaminophen Driven Acute Liver Failure Scientific Reports