Does Acetaminophen Deplete Glutathione? Understanding the Connection Cymbiotika CYMBIOTIKA Can Tylenol (acetaminophen) deplete glutathione levels in the body? NAPQI Wikipedia Early life acetaminophen exposure, glutathione S transferase genes, and development of adolescent asthma in a high risk birth cohort Journal of Allergy and Clinical Immunology Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors.