The Metabolism of Acetaminophen and the Synthesis of Glutathione Download Scientific Diagram Adaptation to acetaminophen exposure elicits major changes in expression and distribution of the hepatic proteome Scientific Reports Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Tylenol And Glutathione Invigor Medical Tylenol, Autism, And The Glutathione Connection OC Integrative Medicine