Perinatal exposure to paracetamol: Dose and sex dependent effects in behaviour and brain's oxidative stress markers in progeny ScienceDirect Acquired pyroglutamic acidosis due to long term dicloxacillin and paracetamol use BMJ Case Reports Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. A 48 hour fast can turn standard Paracetamol into acute liver failure. Fasting depletes Glutathione, leaving the liver defenseless against toxic NAPQI. Review the latest guidelines on BMJ Best Practice: https: bestpractice.bmj.com topics en us 1010