A Possible Antidote for Tylenol (Paracetamol) Overdose American Council on Science and Health Is Tylenol Safe During Pregnancy? Understanding Risk Factors Seeking Health g glutamyl cycle. Depletion of glutathione by acetaminophen leads to Download Scientific Diagram Glutathione, Alcohol and drugs Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors.