Tylenol Avoid or Not to Avoid? Acetaminophen (Tylenol) can deplete glutathione, your body's most important detox antioxidant. This matters even more if your child has an MTHFR gene mutation, which Metabolic processing flowchart of acetaminophen [9, 10]. General Download Scientific Diagram Does Acetaminophen Deplete Glutathione? Understanding the Connection Cymbiotika CYMBIOTIKA Glutathione, Tylenol, and Vaccines: What Parents Need to Know Lotus Homeopathy, Inc. Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors.