Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Amazon.com: Pattern Wellness Glutathione Supplement Active Reduced Glutathione Formula with Added Vitamin C Cell, Antioxidant, + Immune Health Support 30 Vegan Capsules : Health & Household How NSAIDs Affect Glutathione Levels Pure TheraPro Rx Tylenol and glutathione, actually explainedhere's the bullet points: * Lots of concern raised on social media re: glutathione depletion and acetaminophen use * Glutathione is used in the metabolism of acetaminophen and Current Status of Aspirin and Acetaminophen Intoxication Pediatric Annals